A Solution for Liver Testing for 10 Dollars

Brianna Wronko (left) and Guyrandy Jean-Gilles (right)

One of the Penn Bioengineering Department’s senior projects was the work of a two-person team: Brianna Wronko and Guyrandy Jean-Gilles. The result of their work was the MultiDiagnostic, a microfluidics platform that the two students describe as “A Fast, Inexpensive, and Accessible Diagnostic Solution.”

Brianna says that the project was originally conceived as a way for HIV clinics and treatment centers to test biological parameters such as viral load. However, the inability of Brianna and Guy to handle HIV-infected blood in the lab, as well as the desire to generate a product that could both serve patients directly and have a commercial focus. They decided their first offering would consist of liver function tests.

Manufactured by an automated process, the MultiDiagnostic is a paper microfluidics platform with a software component that can be run on a computer or cell phone. When a bodily fluid is placed into the platform, it diffuses into separate chambers of the platform, where colorimetric analysis is then conducted and data communicated via the software’s graphical user interface to the user.

The students currently have the platform in preclinical trials for the testing of aspartase aminotransferase and alkaline phosphatase; the ability to test alanine aminotransferase, bilirubin, and total protein are in the prototyping stage. Their current model is priced at a $10 customer price, which is considerably less expensive than competing technologies already on the market.

Among the most interesting aspects of this senior project team, other than the product itself, was that it had only two members. Asked how this fact affected their work, Brianna admitted that it posed a bit of an obstacle at first. However, she said, “we decided to break up the concept into parts, with me doing the wet lab parts, in which I have a background and Guy, whose background is in software, doing those parts.” In the end, they’re very happy with their final product.